Androgenesis as a Mechanism for Chromosome Set Manipulation in Brook Trout (Salvelinus fontinalis)

May 10, 1988 - BERNIE MAY; KATHRINE J. HENLEY; CHARLES C. KRUEGER; STEVEN P. GLOSS

Journal or Book Title: Aquaculture

Volume/Issue: 75

Page Number(s): 57-70

Year Published: 1988

A variety of parameters were examined to determine the optimal protocol for the induction of androgenesis in brook trout (Salvelinus fontinalis) in order to produce inbred lines. Nuclear DNA in the egg was inactivated with ‘j°Co gamma radiation, the irradiated eggs were fertilized with normal sperm, and the first mitotic division in the newly formed zygote was blocked by hydrostatic pressure to restore diploidy. The optimal 6oCo gamma radiation dosage to inactivate the maternally derived nuclear DNA was 8.8 X lo4 R. The highest percent of embryos reaching the eyed stage occurred when eggs were treated at 8500 psi for 3 min beginning 7.5 h post-fertilization at 10°C. Electrophoretic examination of protein variation in 18 newly emerged sac fry (putative androgens) from four separate treatments revealed all to be of paternal derivation. Nucleoli counts of newly hatched fry were twice as high in pressure treated putative diploids as in untreated putative haploids.

DOI: 0044-8486

Type of Publication: Journal Article

Publisher: Elsevier Science Publishers B.V.

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